AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 19: 3M ™ MDA 2 Salmonella Assay, Presumptive vs. Confirmed – POD Results

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00 0.35

0.00, 0.43 0.18, 0.57

0 7

0.00 0.35

0.00, 0.43 0.00 0.18, 0.57 0.00

-0.43, 0.43 -0.28, 0.28

Instant Non- fat Dry Milk (25 g)

Salmonella Infantis ATCC 51741

0.54 (0.29, 0.91) 3.01 (1.31, 6.89)

20 Hours

20 7

5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00 0.40

0.00, 0.43 0.22, 0.61

0 8

0.00 0.40

0.00, 0.43 0.00 0.22, 0.61 0.00

-0.43, 0.43 -0.28, 0.28

Salmonella Tallahassee ATCC 12002

0.35 (0.16, 0.63) 3.01 (1.31, 6.89)

Black Pepper (25 g)

18 Hours

20 8

5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Test Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00 0.40

0.00, 0.43 0.22, 0.61

0 8

0.00 0.40

0.00, 0.43 0.00 0.22, 0.61 0.00

-0.43, 0.43 -0.28, 0.28

Cocoa Powder (25 g)

Salmonella Newport ATCC 6962

0.55 (0.29, 0.92) 2.29 (1.04, 5.02)

24 Hours

20 8

5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Test Portion Pasteurized Liquid Whole Egg (100 g)

N b

f 95% CI g

Strain

dPOD CP

x c AOAC Research Institute Expert Review Panel Use Only POD CP d 95% CI X POD CC e 95% CI 5 0 0.00 0.00, 0.43 0.34, 0.74 0 0.00 0.55 0.00, 0.43 0.00 0.34, 0.74 0.00 0.68 (0.39, 1.12) 2.62 (1.20, 5.74) 20 11 0.55 11 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00 -

-0.43, 0.43 -0.28, 0.28

Salmonella Enteriditis ATCC 13076

18 Hours

-0.43, 0.43

a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD CP = Candidate method presumptive positive outcomes divided by the total number of trials e POD CC = Candidate method confirmed positive outcomes divided by the total number of trials