AOAC ERP MICRO AUGUST 2018

OMAMAN-31 E: AOAC RI PTM Report 091501 ERP Use Only - March 2016

Table 20: 3M ™ MDA 2 - Salmonella Assay, Presumptive vs. Confirmed – POD Results

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Tes t Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00

0.00, 0.43 0.30, 0.70

0

0.00 0.50

0.00, 0.43 0.00 0.30, 0.70 0.00

-0.43, 0.43 -0.28, 0.28

Raw Whole Shrimp (25 g)

Salmonella Virchow ATCC 51955

0.99 (0.60, 1.69) 4.38 (1.72, 11.15)

18 Hours

20 10 0.50

10

5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Tes t Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00 0.30

0.00, 0.43 0.15, 0.52

0 6

0.00 0.30

0.00, 0.43 0.00 0.15, 0.52 0.00

-0.43, 0.43 -0.27, 0.27

Raw Bagged Spinach (25 g)

Salmonella Schwarzengrun d ATCC STs 95

0.35 (0.17, 0.63) 3.01 (1.31, 6.89)

18 Hours

20 6

5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Tes t Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

-

5

0

0.00

0.00, 0.43 0.34, 0.74

0

0.00 0.55

0.00, 0.43 0.00 0.34, 0.74 0.00

-0.43, 0.43 -0.28, 0.28

Spent Sprout Irrigation Water (375 mL)

Salmonella Saintpaul ATCC 9712

0.68 (0.39, 1.12) 3.82 (1.57, 9.32)

18 Hours

20 11 0.55

11

5

5

1.00

0.57, 1.00

5

1.00

0.57, 1.00 0.00

-0.43, 0.43

Analysi s Time Point

Presumptive

Confirmed

MPN a / Test Portion

Matrix/Tes t Portion

N b

f 95% CI g

Strain

dPOD CP

x c

d 95% CI

e 95% CI

POD CP

X

POD CC

- 5 AOAC Research Institute Expert Review Panel Use Only 0 0.00 0.40 0.00, 0.43 0.22, 0.61 0 8 0.00 0.40 0.00, 0.43 0.00 0.22, 0.61 0.00 0.44 (0.21, 0.76) 2.64 (1.20, 5.81) 20 8 5 5 1.00 0.57, 1.00 5 1.00 0.57, 1.00 0.00

-0.43, 0.43 -0.28, 0.28

Cooked Breaded Chicken (325 g)

Salmonella Kentucky ATCC 9263

18 Hours

-0.43, 0.43

a MPN = Most Probable Number is calculated using the LCF MPN calculator provided by AOAC RI, with 95% confidence interval b N = Number of test portions c x = Number of positive test portions d POD CP = Candidate method presumptive positive outcomes divided by the total number of trials e POD CC = Candidate method confirmed positive outcomes divided by the total number of trials f dPOD CP = Difference between the candidate method presumptive result and candidate method confirmed result POD values