AOAC ERP MICRO AUGUST 2018

B ird et al .: J ournal of AOAC I nternational V ol . 100, N o . 1, 2017  89

Table 2016.07D. Comparative results for the detection of Listeria in 25 g raw chicken breast fillet test portions by the 3M MDA 2– Listeria method versus USDA/FSIS MLG 8.09 in the collaborative study Uninoculated control

CP a

CC b

C c

R d

C vs R

Matrix/ inoculation level Raw chicken breast fillet

N e

x f

Statistic

POD CP

POD CC

POD C

POD R

dLPOD C vs R

dLPOD CP vs CC

Lab

N x

N x

N x

1 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 2 12 1 0.08 12 1 0.08 12 1 0.08 12 0 0.00 3 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 4 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 5 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 6 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 7 12 1 0.08 12 0 0.00 12 0 0.00 12 0 0.00 8 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 9 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 12 2 0.17 12 0 0.00 12 0 0.00 11 h NA NA NA NA NA NA NA NA NA NA NA NA 12 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 13 12 0 0.00 12 0 0.00 12 0 0.00 12 0 0.00 10 g

0.00 0.08 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00

0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.00 0.00

–0.17

NA

NA

0.00 0.00

0.00 0.00

POD CC

POD C

POD R

dLPOD C vs R

dLPOD CP vs CC

Labs

POD CP

N x

N x

N x

N x

Estimate

All 132 2 0.02 132 1 0.01 132 1 0.01 132 0 0.00

0.01

0.01

 LCL i  UCL j

0.00 0.06 0.12 0.11 0.16 0.00 0.00 0.05 0.12 0.11 0.14

0.00 0.04 0.09 0.08 0.16 0.00 0.00 0.04 0.09 0.08 0.10

0.00 0.04 0.09 0.08 0.16 0.00 0.00 0.04 0.09 0.08 0.10

0.00 0.03 0.00 0.00 0.16 0.00 0.00 0.16 0.00 0.00 0.23

–0.02

–0.03

0.04

0.05

s r

 LCL  UCL

k

s L

 LCL  UCL

s R

 UCL  LCL

l

0.5190

0.4338

0.4338

1.0000

P T

Low inoculum level

Raw chicken breast fillet

1 12 7 0.58 12 7 0.58 12 7 0.58 12 7 0.58 2 12 9 0.75 12 9 0.75 12 9 0.75 12 7 0.58 3 12 10 0.83 12 10 0.83 12 10 0.83 12 5 0.42 4 12 9 0.75 12 9 0.75 12 9 0.75 12 4 0.33 5 12 8 0.66 12 8 0.66 12 8 0.66 12 5 0.42 6 12 10 0.83 12 9 0.75 12 9 0.75 12 6 0.50 7 12 8 0.66 12 6 0.50 12 6 0.50 12 6 0.50 8 12 7 0.58 12 7 0.58 12 7 0.58 12 7 0.58 9 12 8 0.66 12 9 0.75 12 8 0.66 12 7 0.58 12 6 0.50 12 8 0.66 12 6 0.50 12 5 0.42 11 h NA NA NA NA NA NA NA NA NA NA NA NA 12 12 7 0.58 12 7 0.58 12 7 0.58 12 4 0.42 13 12 5 0.42 12 5 0.42 12 5 0.42 12 6 0.50 ERP Use Only 0.00 0.17 0.41 0.42 0.22 0.15 0.00 0.00 0.08 0.08 10 g NA 0.16 –0.08

0.00 0.00 0.00 0.00 0.00 0.08 0.16 0.00

–0.08 –0.16

NA

MPN/test portion

0.00 0.00

POD CC

POD C

POD R

dLPOD C vs R

dLPOD CP vs CC

Labs

POD CP

N x

N x

N x

N x

Estimate 0.66

All 132 88 0.67 132 86 0.65 132 85 0.64 132 64 0.48

0.16 0.04 0.28

0.02

 LCL

0.51

0.58 0.75 0.48 0.42 0.52

0.57 0.73 0.48 0.43 0.52

0.56 0.73 0.48 0.43 0.52

0.40 0.57 0.51 0.46 0.52

–0.10

 UCL 0.83

0.13

s r

 LCL  UCL

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