AOAC ERP MICRO AUGUST 2018
OMAMAN-30 D/ PTM Validation Report 081501 OMA ERP - June 2016 ERP Use Only
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inoculum levels for each environmental surface are presented in Table 7. A summary of POD analyses [13] are presented in Tables 8-15. As per criteria outlined in Appendix J of the Official Methods of Analysis Manual [13], fractional positive results were obtained for all matrices.
Internal Study
Raw Chicken Leg Pieces (25 g) – 28 hour Primary Enrichment
For the low inoculation level of the MDA2 - Listeria monocytogenes assay, there were 10 presumptive positives and 10 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 12 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 5 confirmed positive following the reference method.Detailed results of the POD analyses
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are presented in Tables 8 and 12.
Bagged raw spinach
For the low inoculation level of the MDA2 - Listeria monocytogenes assay, there were 9 presumptive positives and 10 confirmed positives following the ISO 11290-1/A1 reference method confirmation procedure resulting in 1 false negative. There were 10 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the ISO 11290-1/A1 reference method confirmation procedure. There were 4 confirmed positives following the reference method.Detailed results of the POD
analyses are presented in Tables 8 and 12.
Cold smoked salmon
Compared to FDA-BAM
For the low inoculation level of the MDA2 - Listeria monocytogenes assay, there were 10 presumptive positives and 10 confirmed positives following the FDA/BAM Chapter 10 reference method confirmation procedure. There were 7 observed positives for the reference method. For the high inoculation level, there were 3 presumptive positives and 3 confirmed positives following the FDA/BAM Chapter 10 reference method confirmation procedure. There were 5 confirmed positives following the reference method.Detailed results of the POD analyses are For the low inoculation level of the MDA2 - Listeria monocytogenes assay, there were 10 presumptive positives and 10 confirmed positives following the ISO 11290-1/A1 reference method confirmation procedure. There were 9 observed positives for the reference method. For the high inoculation level, there were 3 presumptive positives and 3 confirmed positives following the ISO 11290-1/A1 reference method confirmation procedure. There were 4 confirmed positives following the reference method.Detailed results of the POD analyses are presented in Tables 8 and 12. Compared to ISO 11290-1/A1
AOAC Research Institute Exp rt Review Panel Use Only
presented in Tables 8 and 12.
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