AOAC ERP MICRO AUGUST 2018

AOAC Official Methods Program  Expert Review Panel for Microbiology for Foods and Environmental Surfaces 

OMA 2016.08: LISTERIA MONOCYTOGENES IN A VARIETY OF FOODS AND SELECT ENVIRONMENTAL SURFACES

Was the method's applicability considered?

ER 1 ER 2 ER 3 ER 4 ER 5 ER 6

Yes Yes Yes Yes Yes Yes

Please state the method's applicability statement:

ER 1 Applicable to the detection of Listeria monocytogenes in hot dogs (25 and 125 g), salmon (25 g), deli turkey (25 and 125 g) cottage cheese (25 g), chocolate milk (25 mL), vanilla ice cream (25 g), queso fresco (25 g), bagged raw spinach (25 g), romaine lettuce (25 g),  melon (whole), raw chicken leg pieces (25 g), and raw chicken breast fillet (25 g), as well as on sealed concrete [3M Hydrated Sponge with Dey‐ Engley (D/E) Neutralizing Broth; 225 and 100 mL], stainless steel (3M Hydrated Sponge with D/E Neutralizing Broth; 225 mL), and plastic (high‐density polyethylene; 3M EnviroSwab with Letheen Broth; 10 mL) environmental samples ER 2 Variety of Foods and Select Environmental Surfaces ER 3 Applicable to the detection of Listeria monocytogenes in hot dogs (25 and 125 g), salmon (25 g), deli turkey (25 and 125 g), cottage cheese (25 g), chocolate milk (25 mL), vanilla ice cream (25 g), queso fresco (25 g), bagged raw spinach (25 g), romaine lettuce (25 g), melon (whole), raw  chicken leg pieces (25 g), and raw chicken breast fillet (25 g), as well as on sealed concrete [3M Hydrated Sponge with Dey‐Engley (D/E)  Neutralizing Broth; 225 and 100 mL], stainless steel (3M Hydrated Sponge with D/E Neutralizing Broth; 225 mL), and plastic (high‐density  polyethylene; 3M EnviroSwab with Letheen Broth; 10 mL) environmental samples. for the detection of L. monocytogenes in a variety of foods and on select environmental surfaces, including hot dogs (25 and 125 g), salmon (25 g), deli turkey (25 and 125 g), cottage cheese (25 g), chocolate milk (25 mL), vanilla ice cream (25 g), quesofresco (25 g), bagged raw spinach (25 g), romaine lettuce (25 g), melon (whole),  raw chicken leg pieces (25 g), raw chicken breast fillet (25 g), sealed concrete (sponge, 225 and 100 mL), stainless steel (sponge, 225 mL), and plastic (high‐density polyethylene; EnviroSwab, 10 mL). For the detection of L. monocytogenes in a variety of foods and on select environmental surfaces, including hot dogs (25 and 125 g), salmon (25 g), deli turkey (25 and 125 g), cottage cheese (25 g), chocolate milk (25 mL), vanilla ice cream (25 g), queso fresco (25 g), bagged raw spinach (25 g), romaine lettuce (25 g), melon (whole),  raw chicken leg pieces (25 g), raw chicken breast fillet (25 g), sealed concrete (sponge, 225 and 100 mL), stainless steel (sponge, 225 mL), and plastic (high‐density polyethylene; EnviroSwab, 10 mL). ER 4 ER 5

Applicable to the detection of Listeria monocytogenes in hot dogs (25 and 125 g), salmon (25 g), deli turkey (25 and 125 g), cottage cheese (25 g), chocolate milk (25 mL), vanilla ice cream (25 g), queso fresco (25 g), bagged raw spinach (25 g), romaine lettuce (25 g), melon (whole), raw chicken leg pieces (25 g), and raw chicken breast fillet (25 g), as well as on sealed concrete [3M Hydrated Sponge with Dey‐Engley (D/E) Neutralizing Broth; 225 and 100 mL], stainless steel (3M Hydrated Sponge with D/E Neutralizing Broth; 225 mL), and plastic (high‐density polyethylene; 3M EnviroSwab with Letheen Broth; 10 mL) environmental samples.

ER 6

Were the method's safety concerns addressed prior to the approval for AOAC First Action Official Methods status?

ER 1 ER 2 ER 3 ER 4 ER 5 ER 6 ER 1 ER 2 ER 3 ER 4

Yes Yes Yes Yes Yes Yes Yes Yes No Yes

Is the documentation for the safety evaluation available and completed?

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