AOAC SPIFAN Review Team Meeting Book-July 22, 2015

BVIT-02 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE

Abbott Nutrition Division Simultaneous Determination of Total Vitamin B 6, B 2, B 3 in Infant Formula Products by LC-MS/MS Using Enzymatic Digestion and B 1

d)

Riboflavin Riboflavin is readily soluble in dilute alkaline solutions and is only sparingly soluble in water although the solubility of Riboflavin increases in the presence of aromatic compounds including Niacinamide. Crystalline Riboflavin is stable but in solution it is destroyed by UV radiation and visible light. Aqueous solutions between pH=2 and pH=5 are heat stable whereas alkaline solutions are unstable. Riboflavin is stable in the presence of oxygen. Total Riboflavin activity consists of riboflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) predominately. Aqueous Riboflavin solutions exhibit absorbance maxima at 223 nm, 266 nm, 373 nm and 445 nm. The molecular formula for Riboflavin is C 17 H 20 N 4 O 6 and its molecular weight is 376.37 g/mol. Common synonyms include vitamin B 2 and 7,8-dimethyl-10-ribitylisoalloxazine. The CAS# for Riboflavin is 83-88-5. The chemical structure of Riboflavin is shown below:

H

H

H

CH 2 OH

H 2 C C C

C

OH

OH

OH

H 3 C

N

N

O

N

H 3 C

N

O

e)

Thiamin Chloride Hydrochloride Thiamine Chloride Hydrochloride is readily soluble in water, soluble in glycerol, sparingly soluble in ethanol and insoluble in organic solvents. Thiamine Chloride Hydrochloride powder is hygroscopic. Dilute acidic solutions of Thiamine Chloride Hydrochloride are relatively stable to heat but stability decreases as solution-pH increases. Thiamine, thiamine phosphate and thiamine diphsophate are to the primary components contributing to the total vitamin B1 activity. The molecular formula for Thiamine Chloride Hydrochloride is C 12 H 18 Cl 2 N 4 OS and its molecular weight is 336.27 g/mol.

23