AOAC SPIFAN Review Team Meeting Book-July 22, 2015

BVIT-02 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE

Abbott Nutrition Division Simultaneous Determination of Total Vitamin B 6, B 2, B 3 in Infant Formula Products by LC-MS/MS Using Enzymatic Digestion and B 1

Common synonyms include vitamin B 1

, Metatone, Aneurin, Anti-beriberi factor, Biamine,

Betalin S, Betamin, BetaSol, Thiamine and Thiamine Chloride. The CAS# for Thiamine Chloride Hydrochloride is 59-43-8. The chemical structure of Thiamine Chloride Hydrochloride is shown below:

HCl .

NH 2

CH 3

Cl -

N

N

OH

S

H 3 C

N

f) Niacin Niacinamide is the physiologically active form of niacin. Niacinamide (nicotinamide) functions metabolically as a component of the coenzyme nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). Both NAD and NADP function as proton donors and acceptors in metabolic processes including glycolysis, fatty acid synthesis, and respiration. Naturally occurring nicotinic acid is readily converted in-vivo to the biologically active niacinamide. The aqueous solution is neutral to litmus. One gram of niacinamide is soluble in about 1 mL of water and in about 1.5 mL of alcohol. Niacinamide is the form of niacin present in Abbott Nutrition water-soluble vitamin premixes. The molecular formula of niacinamide is C 6 H 6 N 2 0 and its molecular weight is 122.12 g/mol. The CAS# for niacinamide is 98-92-0. Synonyms include: 3-pyridine carboxylic acid amide, 3-pyridinecarboxamide, nicosan 2, nicota, nicotamide, nicotililamido, nicotine acid amide, nicotinamide, nicotinic acid amide, nicotinic amide, nicotol, nicotylamide, nicovit, nicovitol, nicozymin, niko-tamin, Ni-nicotyl, niocinamide, niozymin, pelmin, pelonin amide, pp-faktor, pyridine-3-carboxylic acid amide, savacotyl, vitamin B 3 , vitamin PP, witamina pp, amide pp, aminicotin, amixicotyn, amnicotin, austrovit pp, benicot,

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