AOAC SPIFAN Review Team Meeting Book-July 22, 2015

BVIT-02 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE

Abbott Nutrition Division Simultaneous Determination of Total Vitamin B 6, B 2, B 3 in Infant Formula Products by LC-MS/MS Using Enzymatic Digestion and B 1

c) Bottle-Top Dispenser: Capable of dispensing volumes of 25.0mL ( ± 1mL). Example: Brand Tech Dispensette III, or equivalent. d) Vortex Mixer: Vortex-Genie, or equivalent. e) Laboratory Refrigerator f) Laboratory Freezer g) Magnetic Stir Plate: Variomag multipoint electronic magnetic stir-plate, or equivalent. h) Room Light Shields: A.L.P. Protect-A-Lamp, UV cutoff at 460 nm. i) Volumetric Flasks: Various sizes including 5-mL, 10-mL 25-mL, 50-mL, 100-mL, 250-mL, 500- mL, 1000-mL and 2000-mL. j) Disposable Pasteur Pipettes k) Amber Bottles: Volume capacity of 50 or 100 mL. l) Self-Standing Polypropylene Tubes: Cryovials; 5-mL and 10-mL volume capacity. m) Weighing Vessels: Various including disposable weighing boats and glass weighing funnels. n) Positive Displacement Pipettes: Gilson Microman 10, 25, 100, 250, and 1000 µL. o) Positive Displacement Pipette Tips: Gilson Capillary Piston 10, 25, 100, 250, and 1000 µL. p) Plastic Syringes. B&D 3-mL, or equivalent. q) Syringe Filters: PTFE 0.20µm syringe filters, Acrodisc 25-mm, or equivalent. r) 50-mL Self-Standing Transport Tubes: Superior Scientific, Ltd., or equivalent. s) Autosampler Vials: Waters autosampler vials; 9-mm amber with screw top 12x32 mm pre-split PTFE-silicon septa; Waters Part # 186000847C. 2. Instrumentation NOTE: The following instrumentation has been specifically demonstrated as adequate for the implementation of this method. Comparable instrumentation may be substituted with sufficient qualification. a) Liquid Chromatograph (LC): Waters Acquity Ultra Performance Liquid Chromatograph (UPLC). b) Chromatographic Columns: 2.1 x 50 mm, 1.7-µm Waters BEH C-18Column, and 2.1 x 5mm Vanguard Column. c) Prefilter for Waters Acquity UPLC Column. d) Waters Acquity UPLC 10µL Sample Loop. t) Tamdem Quadrupole Mass Spectrometer (MS/MS) equipped with an Electrospray Ionization (ESI) Source: Waters Quattro Premier XE or equivalent. e) IBM Personal Computer or equivalent. Suitable for operation of the chromatography data system software specified below. 26