AOAC SPIFAN Review Team Meeting Book-July 22, 2015

BVIT-02 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE

Abbott Nutrition Division Simultaneous Determination of Total Vitamin B 6, B 2, B 3 in Infant Formula Products by LC-MS/MS Using Enzymatic Digestion and B 1

b) Mass Spectrometer instrument configuration and operating parameters are provided below.

Table 2. Mass spectrometer configuration and operation parameters Instrument Quattro Premier XE Ionization Mode ESI+ (Electrospray; Positive Ion) Capillary Voltage 3.50 Cone Voltage Various Extractor (V) 5.0 RF Lens (V) 0.0 Source Temperature 120 ºC Desolvation Temperature 325 ºC Cone Gas Flow 50 L/Hr Desolvation Gas Flow 800 L/Hr LM 1 (Low Mass) Resolution 10 LM 2 (Low Mass) Resolution 10 Ion Energy 1 0.2 Entrance Lens 1 Collision Energy Various Exit Lens 1 HM 1 (High Mass) Resolution 10 HM 2 (High Mass) Resolution 10 Ion Energy 2 1.0 Multiplier 710 Collision Cell Pressure ~4 e-3 These parameters are guidelines for the operation of the Quattro Premier XE. These parameters may be changed at the discretion of a qualified LC-MS/MS scientist and the approval of the method supervisor based on the instrument performance provided all system suitability and control sample parameters are met.

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