AOAC SPIFAN Review Team Meeting Book-July 22, 2015

BVIT-02 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE

Abbott Nutrition Division Simultaneous Determination of Total Vitamin B 6, B 2, B 3 in Infant Formula Products by LC-MS/MS Using Enzymatic Digestion and B 1

D.

CHEMICALS, STANDARDS AND SOLUTIONS 1. Chemicals/Standards

a) 2 H 3 b) 2 H 3 c) 13 C 4

-Pyridoxal: Isosciences; Catalog # 7098. -Pyridoxamine: Isosciences; Catalog # D-7099.

-Pyridoxine: Pyridoxine:HCl (4,5-BIS(hydroxymethyl)- 13 C 4

); Cambridge Isotope

Laboratory; Catalog # CLM-7563-0

d) 13 C 3

-Thiamine: Thiamine Chloride (4,5,4-methyl- 13 C 3

); Cambridge Isotope Laboratory; Catalog

# CLM 7667-0.

13 C 4

, 15 N 2

e) f)

-Riboflavin: IsoSciences

2 H 4 -Niacin: CDN Isotopes; Catalog # D-3457. g) 2 H 4 -Nicotinic acid: CDN Isotopes; Catalog # D-4368. h) Niacinamide: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog # 1462006. Store as indicated on label. i) Niacin (nicotinic acid): USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog # 1461003. Stored as indicated on label. j) Pyridoxine Hydrochloride, USP Reference Standard #1587001, current lot. Store as indicated on label. k) Pyridoxal hydrochloride, Sigma P9130 l) Pyridoxamine dihydrochloride >99%, Fluka 82885 m) Riboflavin: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog # 1603006. Store in desiccator protected from white light. Dry according to manufacturer’s instructions prior to use. n) Thiamine Hydrochloride: USP Reference Standard (U.S.P.C., Inc. Rockville, MD) Official Lot; Catalog # 1656002. Store in desiccator protected from white light. Measure the moisture content of the powder prior to use. o) Formic Acid: Sigma ACS Reagent Grade, or equivalent. p) Ethanol (Reagent Alcohol): ACS Reagent Grade, or equivalent.

q) Control Sample: current lot. Store frozen. r) Ammonium Formate ACS grade or equivalent. s) Laboratory Water

t) Methanol: Fisher LC-MS/MS Optima grade or EMD Omni-Solve LC-MS grade. NOTE: METHANOL MUST BE LC-MS GRADE. Other grades including HPLC grade are not acceptable for use. u) Papain Purified Pwd, Spectrum chemical (PA125), or equivalent 31