AOAC SPIFAN Review Team Meeting Book-July 22, 2015

BVIT-02 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE

Abbott Nutrition Division Simultaneous Determination of Total Vitamin B 6, B 2, B 3 in Infant Formula Products by LC-MS/MS Using Enzymatic Digestion and B 1

working standards. Target Lynx uses the powder weight, reconstitution weight, sample weight and final weight of the sample entered into MassLynx to calculate analyte concentration in the original sample. As a result this calculated value will be significantly greater than the analyte concentrations in working standard solutions. The response ratio (ratio of peak area of native compound to internal standard) is not affected by the dilution calculations. ii) If the response ratio for a sample is outside of the response ratio for the corresponding compound calibration curve sample analysis must be repeated using an adjusted sample weight such that the level of the native compound produces a response ratio that falls within the calibration range.

44