AOAC SPIFAN Review Team Meeting Book-July 22, 2015

B-VIT-01 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE

Simultaneous Determination of Thiamine HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide), Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant Formula and Adult Nutritionals

CONFIDENTIAL METHOD FOR ERP ASSESSMENT ONLY

CHEMICALS & REAGENTS Equivalents may be used unless otherwise specified. Standard and reagent preparations may be scaled up or down as needed. No. Reagent (specify grade, if applicable) Concentration Preparation No. Brand Shelf life Storage 1 Formic acid (CH 2 O 2 ), AR 98% N/A Merck as per COA Room temperature 2 Hydrochloric Acid (HCl), AR 37% N/A Merck as per COA Room temperature 3 Hepafluorobutyric acid (HFBA) 98% N/A Sigma as per COA Room temperature 4 Methanol (MeOH; CH 3 OH), HPLC Grade 100% N/A J.T. Baker as per COA Room temperature 5 Acetronitrile (ACN; CH 3 CN) 100% N/A J.T. Baker as per COA Room temperature 6 Ammonium formate, AR ≥ 99% N/A Fluka as per COA 2-8  C 7 Ammonium hydroxide (NH 4 OH), AR 28-30% N/A Merck as per COA Room temperature 8 Isotope Thiamine chloride (Vitamin B1 HCl) (4,5,4-Methyl-13C3, 99%) (*C3C9H17ClN4OS) ≥ 98% N/A CIL Until exhausted or evidence of contamination -20 to-30  C Protect from light, air and moisture.

9

Isotope Riboflavin (Vitamin B2) (13C4, 99%; 15N2, 98%) (C13*C4H20N2*N2O6) Isotope Nicotinamide (D4, 98%) (C6D4H2N2O)

N/A

CIL

Until exhausted or evidence of contamination Until exhausted or evidence of contamination Until exhausted or evidence of contamination Until exhausted or evidence of contamination Until exhausted or evidence of contamination Until exhausted or evidence of contamination Until exhausted or evidence of

Room temperature away from

≥ 98%

light and moisture

10

N/A

CIL

Room temperature away from

≥ 98%

light and moisture

11

Isotope Nicotinic acid (D4, 98%) (C6D4HNO2)

N/A

CIL

Room temperature away from

≥ 98%

light and moisture

12

Isotope Pantothenic acid, Calcium salt monohydrate (beta-alanyl-13C3, 99%; 15N, 98%) Isotope Pyridoxine HCl (4,5-BIS(hydroxyl methyl)-13C4, 99%) (*C4C4H11NO3.HCl) Isotope Pyridoxal:HCl (Methyl-D3, 98%) (C8D3H6NO3.3HCl) Isotope Pyridoxamine:2HCl (Vitamin B6) (Methyl-D3,

N/A

CIL

-5 to 5  C and desiccated. Protect from light. -20 to-30  C Protect from light, air and moisture. Room temp away from

≥ 98%

13

N/A

CIL

≥ 98%

14

N/A

CIL

≥ 98%

light and moisture

15

N/A

CIL

-20  C Protect from light.

≥ 98%

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