AOAC SPIFAN Review Team Meeting Book-July 22, 2015
B-VIT-01 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE
Simultaneous Determination of Thiamine HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide), Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant Formula and Adult Nutritionals
CONFIDENTIAL METHOD FOR ERP ASSESSMENT ONLY
solution
evidence of contamination Until exhausted or evidence of contamination Until exhausted or evidence of contamination Until exhausted or evidence of contamination Until exhausted or evidence of contamination Until exhausted or evidence of contamination
36
Pyridoxine stock internal standard solution
32 mcg/ml
10
2-8 C
37
Pyridoxal hydrochloride stock internal standard solution Pyridoxamine dihydrochloride stock internal standard solution Biotin stock internal standard solution Mixed intermediate internal standard solution I Thiamine HCl stock standard solution Riboflavin stock standard solution Nicotinamide stock standard solution Nicotinic acid stock standard solution Pantothenic acid stock standard solution Pyridoxamine stock standard solution Biotin stock standard solution Mixed water-soluble vitamin intermediate standard solution I Mixed water-soluble vitamin intermediate standard solution II Mixed water-soluble vitamin intermediate standard solution III Mixed water-soluble vitamin working standard solution 1-16 Mobile Phase A: 0.04% HFBA-0.05% Formic Pyridoxine stock standard solution Pyridoxal stock standard solution
25 mcg/ml
11
2-8 C
38
20 mcg/ml
12
2-8 C
39
24 mcg/ml
13
2-8 C
40
14
2-8 C
41
200 mcg/ml
15
3months
2-8 C
42
50 mcg/ml
16
3months
2-8 C
43
1000 mcg/ml
17
3months
2-8 C
44
100 mcg/ml
18
3months
2-8 C
45
500 mcg/ml
19
3months
2-8 C
46
174 mcg/ml
20
3months
2-8 C
47
100 mcg/ml
21
3months
2-8 C
48
73 mcg/ml
22
3months
2-8 C
49
100 mcg/ml
23
3months
2-8 C
50
24
3months
2-8 C
51
25
1month
2-8 C
52
26
1month
2-8 C
53
27
Freshly prepared
54
28
2 weeks
Room temperature
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