AOAC SPIFAN Review Team Meeting Book-July 22, 2015

B-VIT-01 FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE

Simultaneous Determination of Thiamine HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide), Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant Formula and Adult Nutritionals

CONFIDENTIAL METHOD FOR ERP ASSESSMENT ONLY

window Resolution tables Scheduled MRM

Quad 1 and Quad 3: Unit, Scan speed = 10 Da/s

Q1 Mass (Da)

Q3 Mass (Da) 84.00 80.05 78.02 52.99 84.00 80.00 78.00 53.00

Time (min)

Analyte

DP (volts)

EP (volts)

CE (volts)

CXP (volts)

128.00 124.00 124.00 124.00 127.00 123.00 123.00 123.00 171.00 168.02 168.02 168.02 224.00 220.00 220.00 220.00 172.00 169.00 169.00 169.00 174.00 170.00 170.00 170.00 268.00 265.00 265.00 265.00 247.00 245.00 245.00 245.00 383.00 377.00 377.00 377.00

2.27 IS Nicotinic acid 2.27 Nicotic acid_1 2.27 Nicotic acid_2 2.27 Nicotic acid_3 2.27 IS Nicotinamide 2.27 Nicotinamide_1 2.27 Nicotinamide_2 2.27 Nicotinamide_3 2.91 IS Pyridoxal 2.91 Pyridoxal_1 2.91 Pyridoxal_2 2.91 Pyridoxal_3 2.93 IS Patothenic acid 2.93 Pantothenic acid_1 2.93 Pantothenic acid_2 2.93 Pantothenic acid_3 3.03 IS Pyridoxamine 3.03 Pyridoxamine_1 3.03 Pyridoxamine_2 3.03 Pyridoxamine_3 3.30 IS Pyridoxine 3.30 Pyridoxine_1 3.30 Pyridoxine_2 3.30 Pyridoxine_3 4.25 IS Thiamine HCl 4.25 ThiamineHCl_1 4.25 ThiamineHCl_2 4.25 ThiamineHCl_3

96 86 86 86 78 82 82 82 34 46 46 46 12 35 35 35 28 25 25 25 25 47 47 47 40 22 22 22 56 30 30 30 49

10 10 10 10

29 27 29 41 28 38 29 41 20 15 31 25 20 20 17 19 17 19 26 45 19 29 38 47 20 21 20 43 21 21 37 39 31 33 48 50

12 10 10

8

3 4 4 4 3

11

4

10

7 2

153.00 150.10 94.00 122.02 94.00 90.00 202.00 184.00 155.00 152.00 134.00 77.00 156.00 152.00 134.00 77.00 122.00 122.00 144.00 81.00 229.00 227.20 123.00 97.00 249.00 243.00 172.00 198.00

10 10 10

10

8

16 12 11 20 21 19 10 13 20 16 11 15 14 19 16 15 15 22 20 10 14 8 3 9 9

3 5 5 5 8

13 13 13 10 10 10 4

5 4 4 4

6.24 IS Biotin 6.24 Biotin_1 6.24 Biotin_3 6.24 Biotin_2

10 10 10 10

6.54 IS Riboflavin 6.54 Riboflavin_1 6.54 Riboflavin_2 6.54 Riboflavin_3

3 5 5 5

145 145 145

Analyte_1 is for Quantitation.Analyte_2 and 3 for confirmation.

Parameter

Period 1 Experiment 1 CUR

20.00

IS

5500.00 500.00

TEM GS1 GS2 CAD

80.00 80.00

Medium

Valco value Diverter

No.

Total Time (min

Position A-Waste B-MSMS A-Waste

1 2 3

0.0 2.0 7.0

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