AOAC SPIFAN Review Team Meeting Book-July 22, 2015
BVIT-01 (SLV) FOR ERP/WORKING GROUP USE ONLY DO NOT DISTRIBUTE
Method Validation Report
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Validation Report for the Determination of Thiamin HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide), Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant Formula and Adult Nutritionals by LC-MS/MS
Document Title
Table 18B: Ruggedness of Stage 3 Growing up milk formula powder
Concentration (mcg/100g)
Vit B6 (as Pyridoxine)
Pyridoxine Pyridoxal Pyridoxamine
Biotin 14.11 13.70
Day 1 Day 2 Day 3
1094.41 1193.98
107.34 113.60
13.39 13.89
1216.51 1322.93
1122.37
111.53
13.25
1248.58
14.73
Day 4
1148.26
111.87
13.97
1275.53
13.26
Day 5 Day 6 Mean
1083.26 1103.16 1124.24
110.59 111.61 111.09
13.38 13.92 13.63
1208.65 1230.12 1250.39
14.90 14.33 14.17
% RSD
3.66
1.87
2.39
3.43
4.38
Acetance Criteria Accetable (Y/N)
≤5%
≤5%
≤5%
≤5%
≤5%
Y Y Remark: Vitamin B6 (as Pyridoxine) is sum of Pyridoxine, Pyridoxal and Pyridoxamine. All data have been mass corrected and expressed as Pyridoxine. Y Y Y
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