AOAC SPIFAN Review Team Meeting Book-July 22, 2015

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Method Validation Report

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Validation Report for the Determination of Thiamin HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide), Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant Formula and Adult Nutritionals by LC-MS/MS

Document Title

Table 20A: Ruggedness of Partially hydrolyzed protein infant formula powder

Concentration (mcg/100g)

Thiamine HCl

Pantothenic acid

Riboflavin Nicotinic acid

Nicotinamide

Niacin

Day 1 Day 2

620.71

1071.96

206.86

7513.77

7720.63

3445.36

612.51

1003.50

200.64

6995.54

7196.17

3454.24

Day 3

605.50

1109.83

215.92

7344.98

7560.9

3448.02

Day 4

578.27

1031.76

224.52

7493.67

7718.19

3537.58

Day 5 Day 6 Mean

602.73 577.33 599.51

1023.17 1016.23 1042.74

215.10 226.18 214.87

7490.49 7975.53 7469.00

7705.6 8201.71 7683.87

3750.07 3719.42 3559.12

% RSD

2.99

3.86

4.60

4.23

4.21

3.95

Acetance Criteria Accetable (Y/N)

 5%

 5%

 5%

 5%

 5%

 5%

Y Y Remark: Niacin is sum of Nicotinic acid and Nicotinamide, and reported as Niacin. Y Y Y Y

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