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Method Validation Report
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Validation Report for the Determination of Thiamin HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide), Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant Formula and Adult Nutritionals by LC-MS/MS
Document Title
Table 29: Accuracy of Pyridoxamine in Stage 3 Growing up milk formula powder
Pyridoxamine (mcg/100g)
Un- spiked Result
Resulting Spiked Conc
Over Spike
Spiked Result
% Recovery
Replicates
Acceptable
Theoretical Spiked Conc (C)
(A)
(B)
(A+C)
B/(A+C)
Y/N
Replicate 1 14.70
Mean
Replicate 2 13.07
14.01
0%
Replicate 3 14.26
Replicate 1
20.04
21.51
7.50
93.19
Y
50%
Replicate 2
20.98
21.51
7.50
97.57
Y
Replicate 3 Replicate 1 Replicate 2
20.96 28.57 28.62
21.51 29.00 29.00
7.50
97.45 98.51 98.67
Y Y Y
15.00 15.00
100%
Replicate 3
28.05
29.00
15.00
96.72
Y
Acceptance Criteria: All individual recovery results should be within 90% to 110%.
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