AOAC SPIFAN Review Team Meeting Book-July 22, 2015
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Method Validation Report
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Validation Report for the Determination of Thiamin HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide), Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant Formula and Adult Nutritionals by LC-MS/MS
Document Title
Table 32: Accuracy of Riboflavin in Stage 1 Infant formula powder(milk-based)
Riboflavin (mcg/100g)
Un- spiked Result
Resulting Spiked Conc
Spiked Result
% Recovery
Over Spike
Acceptable
Theoretical Spiked Conc (C)
Replicates
(A)
(B)
(A+C)
B/(A+C)
Y/N
Replicate 1
1121.57
Mean
Replicate 2
1126.66
1114.49
0%
Replicate 3
1095.24
Replicate 1
1480.16 1528.39
413.90
96.84
Y
Replicate 2
1459.42 1528.39
413.90
95.49
Y
40%
Replicate 3
1443.36 1528.39
413.90
94.44
Y
Replicate 1
1834.50 1942.29
827.80
94.45
Y
80%
Replicate 2
1883.70 1942.29
827.80
96.98
Y
Replicate 3
1812.29 1942.29
827.80
93.31
Y
Acceptance Criteria: All individual recovery results should be within 90 % to 110 %.
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