AOAC SPIFAN Review Team Meeting Book-July 22, 2015
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Method Validation Report
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Validation Report for the Determination of Thiamin HCl, Riboflavin, Niacin (Nicotinic Acid and Nicotinamide), Pantothenic acid, Vitamin B6 (Pyridoxine, Pyridoxal and Pyridoxamine) and Biotin in Infant Formula and Adult Nutritionals by LC-MS/MS
Document Title
Table 40: Accuracy of Thiamine HCl in Partially hydrolyzed protein infant formula powder
Thiamine HCl (mcg/100g)
Un- spiked Result
Resulting Spiked Conc
Spiked Result
% Recovery
Acceptable
Over Spike
Theoretical Spiked Conc (C)
Replicates
(A)
(B)
(A+C)
B/(A+C)
Y/N
Replicate 1 542.10
Mean
Replicate 2 579.72
572.38
0%
Replicate 3 595.33
Replicate 1
902.75
920.09
347.71
98.11
Y
Replicate 2
891.88
920.09
347.71
96.93
Y
50%
Replicate 3
893.89
920.09
347.71
97.15
Y
Replicate 1
1254.58 1267.80
695.42
98.96
Y
Replicate 2
1275.84 1267.80
695.42
100.63
Y
100%
Replicate 3
1239.66 1267.80
695.42
97.78
Y
Acceptance Criteria: All individual recovery results should be within 90 % to 110 %.
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