APBI 2016

70-gene signature

21-gene signature PAM50

Genomic grade HOXB13:IL17BR 2-gene ratio

11-gene assay

MammaPrint™ (Agendia)

Oncotype DX® (Genomic health)

Prosigna (Nanostring)

MapQuant DX (Ipsogen/Haliodx) Microarray / qRT- PCR

BCI (biotheranostics)

Endopredict® (Sividon)

Microarray

qRT-PCR

qRT-PCR

RT-PCR

qRT-PCR

RS = 0 -100 High / Low / Intermediate

ROR= 0 - 100 High / Low / Intermediate Molecular subtype

0 - 10 High / Low / Intermediate

0 -15 High vs Low

High vs Low

High / Low Equivocal

50 genes

97 genes Simplified : MYBL2, KPNA2, CDC2, CDC20

ER, PR, BCL2, SCUBE2, Ki67, STK15, BIRC5, CCNB1, MYBL2, HER2, GRB7, MMP11, CTSL2, GSTM1, CD68, BAG1 → 10 yr recurrence ER+, N0, with HT ER+, 1-3 N+ FFPE

HOXB13, IL17BR, BUB1, CENPA, NEK2, RACGAP1, RRM2

DHCR7, AZGP1, MGP, STC2, BIRC5, UBE2C, RBBP8, IL6ST

Cryo / FFPE → M+ 5 yr ER+ / ER- N- / N+ (1-3)

FFPE

Cryo / FFPE

FFPE

FFPE

→ 10 yr recurrence HR+ with tamoxifen → 15 yr recurrence

→ recurrence ER+ with tamoxifen

→ 5 yr / 10 yr recurrence

→ 10 yr recurrence ER+, HER2-, with HT N+

adjuvant CT (high)

CT adjuvant / neoadjuvant (high)

HT adjuvant and CT neoadjuvant (high)

HT adj > 5 yr (high)

MINDACT

TAILORx RxPONDER

ASTER 70s