Gluten in Oats Working Group Meeting Book (March 12, 2019)

442–450(2010)]. This definition is not meant to be restrictive and encompasses other related binding‐ 31  based technologies.  32  33  Gluten  [Codex STAN 118‐1979: STANDARD FOR FOODS FOR SPECIAL DIETARY USE FOR PERSONS  34  INTOLERANT TO GLUTEN CODEX STAN 118e_1979: Adopted in 1979. Amendment: 1983 and 2015.  35  Revision: 2008 ].—Protein fraction from wheat, rye, barley, or their crossbred varieties and derivatives  36  thereof, to which some persons are intolerant and that is insoluble in water and 0.5 M NaCl.  37  38  Limit of detection  (LOD; Appendix M).—Lowest concentration or mass of analyte in a test sample that  39  can be distinguished from a true blank sample at a specified probability level.  40  41  Limit of quantitation  (LOQ; Appendix M).—Lowest level of analyte in a test sample that can be  42  quantified at a specified level of precision.  43  44  Recovery  (Appendix F: Guidelines for Standard Method Performance Requirements (Section A2) , Official  45  Methods of Analysis of AOAC INTERNATIONAL  (2019) 21st Ed., AOAC INTERNATIONAL, Rockville, MD,  46  USA ( http://www.eoma.aoac.org/app_f.pdf ) .— The fraction or percentage of analyte that is recovered  47  when the test sample is analyzed using the entire method.  48  49  Repeatability  (ISO 5725‐1).—Variation arising when all efforts are made to keep conditions constant by  50  using the same instrument and operator (in the same laboratory) and repeating during a short time  51  period. Expressed as the repeatability standard deviation (SDr); or % repeatability relative standard  52  deviation (%RSDr).  53  54  Reproducibility  (ISO 5725‐1).—Variation arising when identical test materials are analyzed in different  55  laboratories by different operators on different instruments. The standard deviation or relative standard  56  deviation calculated from among‐laboratory data. Expressed as the reproducibility standard deviation  57  (SDR); or % reproducibility relative standard deviation (%RSDR). 

58  59  60  61  62

5. Method Performance Requirements 

See Table 1.