SIMULTANEOUS DETERMINATION OF 13-CIS AND ALL-TRANS VITAMIN A PALMITATE (RETINYL
PALMITATE), VITAMIN A ACETATE (RETINYL ACETATE), AND TOTAL VITAMIN E (Α-TOCOPHEROL
AND DL-Α-TOCOPHEROL ACETATE) IN INFANT FORMULA AND ADULT NUTRITIONALS BY
NORMAL PHASE HPLC
CODE:
DATE:
PAGE:
VER:
Purpose:
AOAC 2012.10
Jan 2014
1 of 11
04
AOAC MLT Study
Page: 9 of 11
Retinyl Palmitate and Retinyl Acetate
For the purpose of this MLT the trans and 13-cis isomers need to be reported separately.
To do this the 13-
cis
-isomer is calculated with the same slope and intercept as is used to
quantify the trans isomer. This can be done by the HPLC software or using an excel
spreadsheet.
Report the 13-Cis and trans retinyl palmitate and 13-cis and trans retinyl acetate separately,
as mcg/100g for powder and mcg/100mL for liquid presentations.
dl-α-tocopherol and dl-α-tocopherol acetate
dl-α-tocopherol and dl-α-tocopherol acetate are reported separately. Integration by height is
recommended.
Report dl-α-tocopherol and dl-α-tocopherol acetate separately as mg/100g for powder and
mg/100mL for liquid presentations.
Figure 1: Vitamin A palmitate and vitamin A acetate calibration standard.
Vitamin A & E - MLT
For ERP Use Only
DO NOT DISTRIBUTE
Page 9
