531 / 1195
5
Yes
6
No
7
Yes
Reviewer #
8.
Comments:
1
Page 5. Line 9 and Page 12, Line 15: Where is the reference to soft Mexican cheese? Is queso fresco
soft Mexican cheese?
Line 44: Change statement to read "2 unconfirmed positive results" or "2 false positive results".
What is an "unconfirmed false positive?
Page 9, Line 46: you cannot confirm a negative in this situation!. Results are unconfirmed!
2
1. Reference 1 Correct spelling of "Hitchins"
2. Page 1, line 1 of Introduction: Change 6 species to 10 species.
3. Figures are redundant just highlight the relevant data in the Tables using bold type.
3
1. There are no specific cautions about Listeria monocytogenes within the method protocol.
2. Page 3, line 37-the MPN method used should be referenced.
3. Page 3, line 41-Please delete AOAC from the MPN Calculator-there is no reference to AOAC at this
website.
4. Page 4, line 32-the range of hours for incubation instead of just 48 hours should be added.
5. Page 4, line 45-This sentence is awkward-suggest rewording-"A confidence interval of a dLPOD
not containing zero would indicate a statistically...."
6. Page 7, line 2-there is a minor statement about safety precautions, but Listeria monocytogenes
warrants a specific safety statement in this protocol.
7. Page 9, line 44-reword to say....indicating 2 unconfirmed false positive results."
8. Page 13, line 15-delete AOAC and add LCF to the MPN Calculator (there is no reference to AOAC
at this link)
9. Page 14, Table 1-Lab 14 indicates there was no testing, but the other tables and writeup present
data for Lab 14. Should this be Y instead of N?
10. Table 2-display the full name for the ATCC
11. Tables 2013.2A-2D-spell out abbreviations (LCL, UCL)
4
5
none
6
1. Table 4 (Page 16): For Lab # 11 under the AOAC 993.12 Table, there is no superscipt (g) to indicate
that the data for that lab was excluded from statistical analysis.
2. For the pre collaborative study, the requirement for 3 contamination levels was not satisfied (only
uninoculated and low level inoculated samples were tested).
3 Page 46, Last para (lines 28-36): there is no discussion on how the 125 g samples were inoculated.
4. Ideally the Cantaloupe should have been inoculated on more surfaces rather than on the blossom.
Contamination of any area on the Cantaloupe surface is possible.
7
RECOMMENDATION:
Reviewer #
Do you recommend that the ERP adopt this method as an AOAC
Official Methods of
Analysis (First Action status)
1
With editorial changes as indicated
2
Yes.
3
Yes
7
21 June 2013