Page 55 - Alpha Tech Pet, Inc. - Distributor Support Binder (April 2013)

ALPHA TECH PET, INC.

www.alphatechpet.com

119 Russell Street, Suite 21 • Littleton, MA. 01460 (978) 486-3690 • Fax: 486-3693

Relative to the growth of microorganisms on cat urine (which is much stronger and concentrated than dog

urine), the graph below shows the ability of the proprietary microbial complex found in OdorPet® (Freshen

Plus) to grow on cat urine. This growth study was completed using a kinetic plate reader and 96 well BDOBS

(Becton Dickinson Oxygen Biosensor System)

plates. BDOBS plates have a hydrophobic gel with a

fluorescent dye that is quenched in the presence of oxygen. As oxygen is utilized during microbial growth

(which happens when the microbial population feeds on its substrate) the dye fluoresces and is an indication

of growth on the substrate being tested. The graph flat‐lines at the top indicating the maximum

fluorescence obtainable. Clearly there is a short lag time, followed by rapid growth and then a plateau (max

fluorescence) as the substrate is being consumed.

The basic byproducts of bacterial aerobic respiration (growth in the presence of oxygen) are carbon dioxide

and water along with biomass. This is well known to microbiologists and can be found in microbiological

textbooks and literature therefore it is not something we would test for in the lab. In addition this type of

technology is widely employed throughout waste management facilities around the world in onsite

bioreactors and is commonly recognized and employed as an essential step in waste degradation.

This simple study demonstrates efficacy of OdorPet’s biological activity on cat urine, which is only one of a

number of waste product substrates the proprietary microbial complex utilized in OdorPet is effective upon.