ISPAM September 6 2014 Meeting

Comments on the Detection of Salmonella SMPR

In the purpose at the beginning of the document, it was stated that “on-site

verification, a single-laboratory validation, or a multi-site collaborative study” could be done. If no requirements can be elaborated, then they should be deleted. Same comment for other rows in this table and Table 2.

Table 1

TBD = To be determined. Section updated with acceptable limits indicated in the 2nd draft ISO/FDIS 16140.

Revised in version 14.

Table 1 RLOD = TBD. What does this mean?

Table 1 Last 2 footnotes should read 'Annex V' not' Annex 5'.

Agreed.

Revised in version 14

Define abbreviations. Acceptable minimal detection level or minimum acceptable detection level? Definitions refer to minimum but table says minimal.

Table 1

Agreed. "Minimal" should be "minimum".

Revised in version 14

Table 1 (Footnotes)

Suggest using roman numerals instead of numbers for appendices.

Agreed. Change reference to roman numerals.

Revised in version 14

Table 2 LOD - this abbreviation has not been defined earlier.

Agreed. "LOD" should be "AMDL".

Revised in version 14

Table 2 - footnote

Agreed. Reference to ISO, AOAC and Health Canada inserted.

Specify international guideline(s).

Revised in version 14

Positive Control: The term "low", by itself, is not helpful because it is subject to interpertation. Negative control: Is this meant to be a negative control employing a microorganism, or an uninoculated control? Perhaps both need to be considered. Negative control purpose: Specify that the contamination in 2nd sentence means natural and/or accidental contamination.

The working group decided to eliminate Annex I.

Annex I

Annex I removed.

The working group decided to eliminate Annex I.

Annex I

Annex I removed.

The working group decided to eliminate Annex I.

Annex I

Annex I removed.

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