ISPAM September 6 2014 Meeting

Comments on the Detection of Salmonella SMPR

Inhibition control: This may need to be reworded. Can the sample positively impact the ability to detect? If not, then state that inhibition is what is being controlled for. This appears to be the main content of the document – the provision of a standard list of strains recommended for use. However, where are the culture collection numbers? How are the readers to obtain the strains? What if a different set of strains were used in the validation study? CDC Top 20: Some of the numbers in this column are not centered and FDA should be define.

The working group decided to eliminate Annex I.

Annex I

Annex I removed.

The working group decided not to specify culture collection numbers so that method developers/validation could use their own libraries of serovars and species.

Annex II

No change proposed.

Annex II

Agreed. Justify entries in the columns.

Revised in version 14

Pending. Can be formatted when ready for publication.

All genus and species names should be italicized.

Agreed. All references genus, species and subspecies names should follow convention.

Annex II

Annex II

Footnote 2 - Define acronym CDC

Agreed. Define "CDC".

Revised in version 14

It is accepted practice to italicize all genus, species and subspecies names but not serovar names which should have a capitalized first letter. For example entry 37, 48, and 89 serovars should be "Indiana, Infantis" and "Inverness" respectively. In entries 3, 12, 19 change subspecies name 'Salamae' to 'salamae'. Conventionally 'subsp., spp., sp., var., and serovar.' are not italicized. There would need to be more information for the non enterica subsp organism than just the O group. The past few times that we have done a validation or an inc/exc study reviewers required the antigenic formula for the non enterica subsp.

Annex II

Agreed.

Revised in version 14

Reporting the antigenic formula would be dependent upon the individual strains used in a study and could vary. As long as Salamae is represented as a subspecies, this would still allow method developers to utilize strains from different collections.

No change proposed.

Annex II; #3

This serovar is now recognized as Westhampton var 15+

Annex II; #21

Agreed.

Revised in version 14

If this is the organism from the Mango outbreak this is the correct spelling is Braenderup.

Annex II; #46

Agreed.

Revised in version 14

Annex II, #50 Spelling is Mbandaka

Agreed. Change to "Mbandaka".

Revised in version 14

This should be deleted. It causes fowl typhoid, a severe disease in fowl that is usually tightly controlled due to its animal health implications.

The working group decided that this serovar should remain on the inclusivity panel.

Annex II, #62

No change proposed.

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