CROI 2015 Program and Abstracts

Abstract Listing

Poster Abstracts

pathways (if any) might be over-represented in patients that would later develop IRIS we focused on biological function terms as well as upstream regulators that showed statistical significance of 10 -7 in IPA. Results: From a completed longitudinal cohort of ART-naive patients with CD4 <100 cells/ m L starting ART, PMC were available from 143 patients pre-ART. Patients had a median age of 38 (IQR 30-45) years, CD4 22 (IQR 9-48) cells/ m L and plasma HIV VL 4.99 (IQR 4.45-5.36)log 10 copies/mL. Thirty six of them (25%) developed IRIS, based on ACTG criteria, at a median of 29 (IQR 7-56) days after ART initiation. PCA did not result in clear discrimination of PMC from IRIS versus non-IRIS patients, suggesting that at the time of analysis, before ART initiation, both groups looked similar at the molecular level. The most striking biological theme suggested by IPA analysis was expression of specific gene subsets associated with inflammatory responses and apoptosis/necrosis. Specifically, the IL-10 and IL-1 molecular pathways were highlighted in IRIS samples (Figure).

Poster Abstracts

Differential gene expression for transcripts canonical pathway“IL-10 Signaling”. Literature curated interactions between gene products are portrayed by shapes indicating type of gene product, while colors indicate the magnitude of difference in mRNA expression observed in this study. Transcripts in red were more abundant in IRIS patients, in green were more abundant in non-IRIS. Conclusions: This study highlights possible molecular pathways, such as IL-1 and IL-10, involved in IRIS etiology that might have important implications for IRIS prevention and treatment. In addition, these findings may have implications for the diagnosis and treatment of complications in patients recovering from lymphopenia caused by etiologies besides HIV.

WEDNESDAY, FEBRUARY 25, 2015 Session P-C10 Poster Session

Poster Hall

2:30 pm– 4:00 pm Immune Activation and HIV Pathogenesis 312 Inflammatory Biomarkers Decline but Do Not Normalize After 10 Years of cART Kenneth A. Lichtenstein 1 ; Carl Armon 2 ;Vijaya Knight 1 ; Rafeul Alam 1 1 National Jewish Health, Denver, CO, US; 2 Children’s Hospital Colorado, Aurora, CO, US Background: HIV infection is characterized by a state of chronic inflammation (CI). The degree to which combination antiretroviral therapy (cART) contributes to or ameliorates CI over time is unclear. Methods: We measured levels of several inflammatory biomarkers (IB) in the 10 year period following initiation of cART in 327 patients participating in the Study to Understand the Natural History of HIV Infection in the Era of HAART (SUN Study) who had HIV-RNA levels < 200 copies/ml at each measurement. IB levels were measured in the specimen proximal to initiation of cART in 40 cART-naïve (cART-N) SUN Study patients and in a single specimen obtained from each of 30 case-matched HIV-negative controls (HNC). Baseline characteristics were similar between cART-experienced (cART-E) and cART-N groups. Frozen serum specimens were analyzed for RANTES, TNF-alpha, MIP-1 alpha and beta, MCP-1,

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CROI 2015