AOAC SPIFAN Stakeholder Panel Meeting Book (March 12, 2020)

Working group activities

Discussed during the calls (major topics)

• Analytes?

2’-FL , 3-FL, LNnT , LNT, 6’-SL, 3’-SL, DFL, (LNFP-I)

• SMPR for standards?

not in the scope of SPIFAN and current SMPRs

• Analytical range per HMO?

based on data for breast milk and IF

• Selectivity / interferences?

possible interferences discussed, incl. probiotics

• Reference materials?

not currently available

Specific Performance Claims: 2’-FL Table 1: Method Performance Requirements a Analytical range 5 – 500 mg/100g Limit of Quantitation (LOQ) 4 mg/100g

85-110% (5-20 mg/100g ) 90-110% (>20 mg/100g)

Recovery

Repeatability (% RSD r ) Reproducibility (% RSD R ) a Concentrations apply to: i) “ready-to-feed’ liquids “as is”; ii) reconstituted powders (25 g into 200 g of water); and iii) liquid concentrates diluted 1:1 by weight.