AOAC SPIFAN Stakeholder Panel Meeting Book (March 12, 2020)

Summary of Existing Methods for Vitamin A in OMA, Chapter 50

AOAC Official Method 992.04 Vitamin A (Retinol) in Milk-Based Infant Formula

Analytes: Retinol, 13-cis Retinol

Standard: Retinyl Palmitate

Prep: Saponification and solvent extraction

Normal-phase, Si, 3um

MP: Hexane–isopropyl alcohol (1-5% adjusted, v/v); Flow = 1-2ml/min

Detection: UV 340 nm

Mean recovery = 94.3%; RSDr = 3.62%; RSDR = 9.72%; Range = na

Limitations: 340 nm,100ul inj; >1 ml/min flow;

Summary of Existing Methods for Vitamin A in OMA, Chapter 50

AOAC Official Method 992.06 Vitamin A (Retinol) in Milk-Based Infant Formula

Analytes: Retinol, 13-cis Retinol

Standard: Retinyl Palmitate

Prep: Saponification and solvent extraction

Normal-phase, CN, 5um

MP: Hexane–isopropyl alcohol (100 + 0.25, v/v), Flow = 1.5 ml/min

Detection: UV 336 nm

Mean recovery = 99%: RSDr = 4.9%; RSDR = 10.5%; Range = >15.8 ug/100mL

Limitations: 336 nm,100ul inj; 1.5 ml/min flow; LOD

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