AOAC SPIFAN Stakeholder Panel Meeting Book (March 12, 2020)
Summary of Existing Methods for Vitamin A in OMA, Chapter 50
AOAC Official Method 2002.06 Retinyl Palmitate (Vitamin A) in Fortified Fluid Milk
Analytes: Retinyl Palmitate
Standard: Retinyl Palmitate; Int Std: Retinyl Acetate
Prep: Solvent extraction
Normal-phase, Si, 5um
Detection: UV 325 nm
MP: hexane–isopropanol (99.85 + 0.15), Flow = 1.0 ml/min
Mean recovery = na%: RSDr = 2.3%; RSDR = 7.9%; Range: 44.0–303.0 mg/100 mL
Limitations: 5um, 100 uL inj,
Summary of Existing Methods for Vitamin A in OMA, Chapter 50
AOAC Official Method 2011.07 Vitamin A in Infant Formula and Adult Nutritionals Analytes: trans-Retinol
Standard: trans-Retinol
Prep: Saponification and SPE
UPLC, HILIC, BEH, 1.7um; run as Normal-phase
MP: 1% (v/v) 2-propanol in n-hexane, Flow = 1.2 ml/min, 4 ul inj
Detection: UV 326 nm
Mean recovery = 101%; RSDr = 6.0%; RSDR = 13.4%; Range 9-450 ug/100g
Limitations: single column supplier, limitation of particle size
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