AOAC SPIFAN Stakeholder Panel Meeting Book (March 12, 2020)

Summary of Existing Methods for Vitamin A in OMA, Chapter 50

AOAC Official Method 2002.06 Retinyl Palmitate (Vitamin A) in Fortified Fluid Milk

Analytes: Retinyl Palmitate

Standard: Retinyl Palmitate; Int Std: Retinyl Acetate

Prep: Solvent extraction

Normal-phase, Si, 5um

Detection: UV 325 nm

MP: hexane–isopropanol (99.85 + 0.15), Flow = 1.0 ml/min

Mean recovery = na%: RSDr = 2.3%; RSDR = 7.9%; Range: 44.0–303.0 mg/100 mL

Limitations: 5um, 100 uL inj,

Summary of Existing Methods for Vitamin A in OMA, Chapter 50

AOAC Official Method 2011.07 Vitamin A in Infant Formula and Adult Nutritionals Analytes: trans-Retinol

Standard: trans-Retinol

Prep: Saponification and SPE

UPLC, HILIC, BEH, 1.7um; run as Normal-phase

MP: 1% (v/v) 2-propanol in n-hexane, Flow = 1.2 ml/min, 4 ul inj

Detection: UV 326 nm

Mean recovery = 101%; RSDr = 6.0%; RSDR = 13.4%; Range 9-450 ug/100g

Limitations: single column supplier, limitation of particle size