AOAC SPIFAN Stakeholder Panel Meeting Book (March 12, 2020)

Summary of Existing Methods for Vitamin A & E in OMA, Chapter 50

AOAC Official Method 2012.10. Simultaneous Determination of Vitamins E and A in Infant Formula and Adult Nutritionals

Analytes: Retinyl palmitate (cis and trans), retinyl acetate (cis and trans), -tocopherol, - tocopherol acetate

Standard: Retinyl palmitate, retinyl acetate, -tocopherol, -tocopherol acetate

Prep: Enzymatic hydrolysis and solvent extraction

Normal-phase, NH2 column, 5 um

MP: A = n-Hexane; B = 750 mL n-hexane + 250 mL methyl t-butyl ether + 3 mL methanol

Detection: Vitamin E - fluorescence, excitation/emission, 280/310 nm. Vitamin A – UV, 325 nm

Mean recovery = see tables

Limitations: 5 um column, 50 ul inj, 1.5ml/min flow, not optimum FL wavelength