AOAC SPIFAN Stakeholder Panel Meeting Book (March 12, 2020)

Summary of Existing Methods for Nutrient in OMA, Chapter 50

AOAC

Technology Scope

Meets SMPR?

Remarks

986.23 Microbiological assay

All active forms of cobalamin (some inactive forms as well) Vitamin B12 (as cyanocobalamin) Vitamin B12 vitamers as cyanocobalamin

No

CCNFSDU41 has confirmed use of microbiological methods.

•

• Part of recommended methods (CX234) as Type III method

2011.08 LC-UV

Yes/No

Codex Type II method

•

• Several matrixes show SDR higher than SMPR

2011.09 LC-UV

Yes

Equivalent to 2014.02.

•

• Compares well to current Codex Type II • No full validation data on SPIFAN matrixes available

2014.02 LC-UV

Vitamin B12 (all active forms) as cyanocobalamin

Yes

Equivalent to 2011.09

•

• Compares well to current Codex Type II • Full validation on SPIFAN matrixes (including MLT data) available • Widely used

Conclusions

AOAC

Technology Scope

Meets SMPR?

Remarks

986.23 Microbiological assay

All active forms of cobalamin (some inactive forms as well) Vitamin B12 (as cyanocobalamin) Vitamin B12 vitamers as cyanocobalamin Vitamin B12 (all active forms) as cyanocobalamin

No

CCNFSDU41 has confirmed use of microbiological methods.

•

• Part of recommended methods (CX234) as Type III method • To be kept, although several drawbacks

2011.08 LC-UV

Yes

Codex Type II method

•

2011.09 LC-UV

Yes

Recommend to repeal

•

2014.02 LC-UV

Yes

Could be recommended as Type III

•