SPDS Meeting Book
4. 3’‐Epilutein
o (3R,3’S,6’R)‐lutein o Not dietary— no biological or commercial role o Significant epimer product and loss of lutein o Occurs in aqueous acid o Reaction likely proceeds by S N 1 and S N 2, but mostly S N
2 since
conversion exceeds 50%
o Structure
General Analytical Needs
Method should – Quantitatively de‐esterify all analyte forms – Separate and accurately quantify relevant free analytes • Lutein • Zeaxanthin • β‐Cryptoxanthin • 3’ Epilutein (principal lutein metabolite) ‐ – Determine the above in • Raw materials used in dietary supplement formulations • Finished products?
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