Research Masterclass in Radiotherapy Physics 2017

Methodology

3 human cell lines:

- MRC-5, normal lung fibroblasts - A549, lung cancer, p53 wild-type - NCL-H23, lung cancer, mutant p53

Irradiation:

- broadbeam - microbeam

2D clonogenic assays:

- conventional measure of cell sensitivity to radiation

3D cell culture assays:

- spheroid growth curves - cell viability assay - image changes in spheroid morphology