High-Performance & High-Capacity Centrifuges Catalog

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JE-5.0

JE-5.0

Elutriation

Elutriator Rotor For use in Avanti J-26S XP Series and J6-MI centrifuges equipped with viewport door and strobe assembly. Max. RPM Max . g Maximum Elutriation Volume Boundary 5 000 4 700 40 mL 86 mm (2,410 g )

1

For use in Avanti J-26S XP centrifuge. *

No. 356900 . JE-5.0 Elutriator Rotor Assembly. Includes bypass chamber and supplies, and features a quick-release assembly which can be autoclaved for sterile operation. Order elutriation chamber(s) separately. (For complete Elutriation System, a number of components are necessary. See How to Order below.)

Recommended Pump (not supplied by Beckman Coulter) We recommend the Cole-Parmer Masterflex Digital Drive Pump System. These are not available from Beckman Coulter, but may be ordered directly from Cole-Parmer (625 East Bunker Court, Vernon Hills, Illinois 60061-1844 USA, Phone 847-549-7600, Fax 847-549- 7676, TDD hotline: 800-833-7400, E-mail: info@coleparmer.com). Contact Cole-Parmer directly to receive current ordering information for these or comparable pumps. A complete pump consists of one each of the following components: 1. Easy-Load pump head (stainless steel) 2. Tygon L/S 16 tubing standard drive (10 to 600 rpm, 115 VAC or 230 VAC) Supplies 335148 Silicone Vacuum Grease, 1 oz 339555 Solution 555 Cleaning Concentrate, 1 qt 306812 Spinkote Lubricant (for rotor/shaft interface), 2 oz

Elutriation Chamber Description Part No.

Max. Cells Recovered per Run

Minimum Cells Required for Loading per

Max

Elutriated

Buffer Volume

Fraction

40-mL 5-mL

356940 356943 356945

10 10 10 10 10 10

10 7 10 7 10 5

1000 mL 1000 mL 1000 mL

Sanderson

How to Order

For a complete Beckman Coulter Elutriation System, the following components are required: 1. An Elutriator Rotor Assembly 2. An Elutriation Chamber 3. An Elutriation Centrifuge equipped with viewport door and strobe assembly. Beckman Coulter Avanti J-26S XP centrifuges can be ordered as Elutriation Centrifuges fully equipped with the appropriate door and strobe assembly. See Section 1 for ordering information. Also necessary but not available from Beckman Coulter is a pump that meets the following requirements: (a) has continuously adjustable flow rate from 6 to 100 mL/min; (b) has flow rate controllable to within ± 1.5%; (c) has flow rate independent of back pressure up to 40 psi; and (d) has pulsation less than 5% of flow rate. Recommended pumps are listed below. To Upgrade an Existing Beckman Coulter Centrifuge For Avanti J-26S XP Series, order the appropriate kit listed below (order only one kit): No. 366562 . 50/60 Hz. For use with Avanti J-26S XP. Kit includes Strobe Lamp/Control Assembly and Door Assembly with Viewport.

357520 Tubing, Tygon 1 / 8 -in. (I.D.) × 1 / 4 -in. (O.D.) 357580 Tubing, Silicone 3 / 16 -in. (I.D.) × 5 / 16 -in. (O.D.) (for rotating seal-assembly connection)

Tools 927784 Pliers for Retaining Clip (bearing removal)

016223 Wrench, 1 / 8 -in. Hex Driver 927766 Wrench, 5 / 16 -in. Hex T-handle 029840 Wrench, 5 / 32 -in. Hex Driver 001884 Wrench, 5 / 64 -in. Hex Driver 817305 Wrench, 9 / 64 -in. Hex Angle

* When properly equipped for elutriation.

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Beckman Coulter High Performance Centrifuges