AOAC_SPDS_ERP_-_Set_3

AOAC SPDS ERP - Set 3 Review Form 2

Submission Date

2016-07-25 13:07:52

Name

Nour Eddine ES-SAFI

E-mail

nouressafi@yahoo.fr

Organization

Mohammed V University in Rabat

Title of Method

Analysis of Theanine in Tea (Camellia sinensis) Dietary Ingredients and Supplements by HighPerformance Liquid Chromatography with Post-Column Derivatization: Single Laboratory Validation

AOAC Candidate Method Number (e.g. ALN-01)

TEA-01

Applicable SMPR

2015.014

Summary:

The Tea-1 method presents experimental technique for Theanine analysis in dietary ingredients and supplements using simple buffer extraction followed by cation-exchange chromatography, post-column reaction with Ninhydrin reagent and UV/Vis detection The AOAC SMPR 2015-014 concerns the quantitative determination of catechins, methyl xanthenes, theaflavins, and theanine. In the proposed Tea-1 method only theanine is determined.

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

The analytical technique used in the Tea-1 method meet the SMPR. From all the given results only one does not meet the SMPR. (Recovery: 108.7 % instead of 107 %)

Yes

Yes