AOAC_SPDS_ERP_-_Set_3

2. Does the method contain system suitability tests or controls as specified by the SMPR? If not, please indicate if there is a need for such tests or controls and which ones. 3. Is there information demonstrating that the method system suitability tests and controls as specified in the SMPR worked appropriately and as expected? If no, please specify. 4. Based on the supporting information, is the method written clearly and concisely? If no, please specify the needed revisions. 5. Based on the supporting information, what are the pros/strengths of the method?

Yes. The proposed method contains injections of blank reagent, working calibration solutions and control samples.

Yes

Yes

The Proposed method uses a cation-exchange HPLC separation technique coupled with post-column ninhydrin derivatization. This has been considered as a reference method in term of its robust nature, its quantitative performance and reproducibility. Sample components are separated in the column before the reaction, it is not affected by the sample matrix during reaction with the derivatizing reagent.

6. Based on the supporting information, what are the cons/weaknesses of the method?

Sensitivity may be questionable Relatively high reagent consumption The use of UV-visible detection The purity of the separated peaks should be checked Only one analyte (theanine) is explored

7. Any general comments about the method?

No

Do you recommend this method be adopted as a First Action and published in the Official Methods of Analysis of AOAC INTERNATIONAL? Please specify rationale.

The proposed method meets the major SMPR criteria and then could be adopted (after discussion with the ERP members) as a First Action