AOAC_SPDS_ERP_-_Set_3

AOAC SPDS ERP - Set 3 Review Form 2

Submission Date

2016-07-27 15:55:23

Name

Nour Eddine ES-SAFI

E-mail

nouressafi@yahoo.fr

Organization

Mohammed V University in Rabat

Title of Method

Determination of Catechins and Caffeine in Camillia sinensis Raw Materials, Extracts, and Dietary Supplements by HPLC-UV: Single-Laboratory Validation

AOAC Candidate Method Number (e.g. ALN-01)

TEA-01-02

Applicable SMPR

2015.014

Summary:

The method Tea-2 presents results dealing with the quantification of 7 catechins (C, GC, EC, EGC, GCG, ECG, EGCG) and caffein in green tea raw material, powdered extract and dietary supplements containing green tee extract. Quantitative analysis was performed by RP HPLC separation and UV detection. Yes. The investigated analytes are all indicated in the applicability of the AOAC SMPR 2015.014. In addition to caffein 7 catechins are concerned by the study and included (+)-catechin, galloxcatechin, (-)-epicatechin, epigallocatechin, gallocatechin gallate, epicatechin gallate and epigallocatechin gallate LOQ: not clearly indicated. LOD: not indicated. Reproducibility: not conducted. Recovery: Bad results were obtained in some cases. Repeatability: Good results were obtained with EGC, caffein, EGCG. Bad results were obtained with GC, C, EC, GCG and ECG.

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

Yes

Yes