AOAC_SPDS_ERP_-_Set_3

2. Does the method contain system suitability tests or controls as specified by the SMPR? If not, please indicate if there is a need for such tests or controls and which ones. 3. Is there information demonstrating that the method system suitability tests and controls as specified in the SMPR worked appropriately and as expected? If no, please specify. 4. Based on the supporting information, is the method written clearly and concisely? If no, please specify the needed revisions. 5. Based on the supporting information, what are the pros/strengths of the method? 6. Based on the supporting information, what are the cons/weaknesses of the method?

Yes the method contains system suitability test including duplicate injections of standard solution and calibration standard.

Repeatability results obtained using the SRM 3254 reference material showed many bad data. This is the case of GC (14.9 %), C (10.8 %), EC (5.16 %), GCG (5.72 %) and ECG (5.66 %). The AOAC SMPR 2015.014 indicated teh obtained values should be less or equal to 5.

Repeatability results with the SRM 3255 reference material showed good results.

Repeatability results with the SRM 3256 reference material showed good results except for GC and C where bad values have been obtained (5.57 and 8.94 % respectively).

Yes

The proposed method is rapid, simple and robust. The optimized used chromatographic conditions gave chromatographic profiles where the investigated analytes are separated during a relatively short time.

Not all the method performance requirements are performed. Does not meet the SMPR

7. Any general comments about the method?

No

Do you recommend this method be adopted as a First Action and published in the Official Methods of Analysis of AOAC INTERNATIONAL? Please specify rationale.

I do not recommend this method be adopted as a first action in the present form. I think that more experiments are needed and should concern all the performance requirements indicated in the 2015.014 SMPR.