AOAC_SPDS_ERP_-_Set_3

1. Are the definitions specified in the SMPR used and applied appropriately in the supporting documentation (manuscripts, method studies, etc...)? If not, please explain the differences and if the method is impacted by the difference. 2. Is there information demonstrating that the method meets the SMPR Method Performance Requirements using the Reference Materials stated in the SMPR? If not, then specify what is missing and how this impacts demonstration of performance of the method. method performs within the SMPR Method Performance Requirements using the Reference Materials stated in the SMPR? If not, then specify what is missing and how this impacts demonstration of method performance. 3. Is there information demonstrating that the method performs within the SMPR Method Performance REquirements table specifications for all analytes in the SMPR applicability statement? If not, please specify what is missing and whether or not the method's applicability should be modified. 1. Based on the supporting information, were there any additional steps in the evaluation of the method that indicated the need for any additional precautionary statements in the method? 4. Is there information demonstrating that the

Yes.

No. 3 NIST SRMs were used for demonstration of accuracy. Values reported in the method were compared to the values in the COAs from NIST. Values for GC and GCG were outside of the certified range (high) for all 3 materials. The values for EGCG in the green tea leaves and for ECG in the green tea extract were also high. This indicates a likely interference in the chromatography with GC and GCG, and possibly with the other components as well.

No. 3 NIST SRMs were used for demonstration of accuracy. Values reported in the method were compared to the values in the COAs from NIST. Values for GC and GCG were outside of the certified range (high) for all 3 materials. The values for EGCG in the green tea leaves and for ECG in the green tea extract were also high. This indicates a likely interference in the chromatography with GC and GCG, and possibly with the other components as well.

The method only addresses the determination of 7 catechins and caffeine. The method does not address the determination of CG, other methyl xanthines, theanine, or theaflavins.

The method could potentially be extrapolated to include some of the other compounds (CG, theobromine, theophylline).

No