AOAC_SPDS_ERP_-_Set_3

AOAC SPDS ERP - Set 3 Review Form 2

Submission Date

2016-07-30 04:41:22

Name

Darryl Sullivan

E-mail

darryl.sullivan@covance.com

Organization

Covance Laboratories

Title of Method

Determination of Catechins and Caffeine in Camillia sinensis Raw Materials, Extracts, and Dietary Supplements by HPLC-UV: Single Laboratory Validation.

AOAC Candidate Method Number (e.g. ALN-01)

TEA-02

Applicable SMPR

2015.014

Summary:

This method can quantify seven catechins and and caffeine in green tea raw materials, and powdered extracts and dietary supplements containing green tea extract. The method utilizes reverse phase HPLC with a phenyl-based stationary phase and and gradient elution. Detection is accomplished using UV absorption.

1. Does the applicability of the method support the applicability of the SMPR? If not, please explain what is missing. 2. Does the analytical technique(s) used in the method meet the SMPR? If not, please specify how it differs from what is stated in the SMPR. 3. Are the definitions specified in the SMPR used and applied appropriately in the method? If no, please indicate how the terms are used. 4. Does the method, as written, contain all appropriate precautions and warnings related to the method's reagents, components, instrumentation, or method steps that may be hazardous? If no, please suggest wording or option(s).

This method is capable of measuring catechins and caffeine, but has not been demonstrated to be capable of determining methyl xanthines, theaflavins, or theanine.

The method appears to meet the SMPR for catechins.

yes

yes