Biophysical Society 59th Annual Meeting Program Guide

Room A: Monday, February 9

1:30 pm –3:00 pm World Precision Instruments Side-Stepping the Animal Model: Cardiac Work Loops in Human iPSC-derived Myocytes. Cardiac pressure-volume loops on a complete organ provide the frame- work for understanding cardiac mechanics in experimental animal models, most notably in the context of Frank-Starling mechanisms. With the development of more sensitive transducers, this work has been applied to single cardiac cells, using freshly isolated cells from an animal model. With the advent of iPSC-derived myocytes, a whole new range of cell types is now available to the investigator. We introduce a novel mounting applica- tion for overcoming the technical difficulties in instrumenting these cells for force measurements. With this technology, it is now possible to conduct experiments on human stem cell-derived myocytes. We will show preliminary results, the tools required for these types of experiments, mounting methods, and a novel method for direct force measurements on human iPSC-derived myocytes. In addition, two dif- ferent methods for real-time determination of length changes in isolated iPSC-derived myocytes will be presented. The results are preliminary, however indicate the possibility for not only a reduction in the use of the animal models in cardiac research, but also the direct investigation of human cardiovascular disease. 3:30 pm –5:00 pm Bruker Nano Surfaces Recent Advances in Atomic Force Microscopy for Biological Research Bruker’s latest BioScope AFM is the perfect integration of AFM and invert- ed light microscopy. It incorporates Bruker’s latest Peak Force Tapping innovations including the new nanomechanics package, which signifi- cantly expands mechanobiology applications into a lower modulus range covering live cells and tissues. With its open access design, and bio friendly features and accessories, the latest BioScope AFM is the most integrated and easiest to use life science AFM available. The workshop will include examples of the functional integration of light microscopy techniques with AFM in order to conduct optically guided, high-resolution mapping of both the structural and mechanical properties of mammalian cells. Presenter John Thornton, Senior Applications Engineer, Bruker Nano Surfaces For over 40 years, HEKA has provided innovative products, expert tech support and unmatched service to their customers. HEKA's commitment to technological innovation is reflected by consistent updating of both hardware and software. While yesterday's gold standards try to keep pace with the latest research techniques, HEKA takes the lead. By popular demand, HEKA is hosting a series of user meetings with tutorial presentations. On one hand, some of the new products will be showcased to the experienced user and, on the other hand, step-by-step guidance is provided to the researcher who is new to the field. Registration is available online through the HEKA Events Page on EventBrite, or by 5:30 pm –7:00 pm HEKA Elektronik HEKA Electrophysiology Update

9:30 am –11:00 am Pall ForteBio LLC Measuring Engineered Changes in Binding Affinity with the BLItz® Label-Free System Combining Organic Synthesis and Directed Evolution to Design Glycocluster HIV Vaccine Candidates We will describe a new method for design of carbohydrate HIV vaccines, which combines organic synthesis and directed evolution techniques. This work originates from the observation that some HIV positive individuals produce antibodies which are broadly neutralizing and protective against HIV infection. One such antibody, 2G12, recognizes and binds to a cluster of carbohydrates on the viral envelope protein gp120. Our goal is to develop synthetic carbohydrate clusters which closely mimic the viral carbohydrate cluster, and which might thus elicit a 2G12-like antibody response when used as a vaccine. In order to design carbohydrate clusters which closely mimic gp120, we have developed evolution-based strategies, in which immobilized 2G12 is used to recognize and fish out the best gly- cocluster mimics of gp120 from amongst large libraries of ~10 trillion dif- ferent glycosylated peptide- or DNA structures. The glycocluster structures obtained by these methods are recognized by antibody 2G12 as strongly as is the viral protein itself, and are thus of great interest for vaccine studies. Tips and Tricks for Developing BLItz Assays The BLItz label-free assay system is a simple-to-use benchtop instrument for measuring binding interactions of antibodies and proteins using as little as 4µl of sample. Additional case studies of how the BLItz system is being used to qualify biophysical models will be presented, along with tips and tricks for developing kinetics assays on the BLItz system. Presenters Isaac Krauss, Assistant Professor of Chemistry, Brandeis University Craig Tin, Senior Product Manager, Pall Forte Bio LLC 11:30 am –1:00 pm Asylum Research, an Oxford Instruments Company There’s No Other AFM Like Cypher™ — High Resolution Atomic Force Microscopy Made Easier and Faster Asylum Research has focused on improving AFM instrumentation to make imaging in liquid easier, faster and more quantitative for life sci- ence applications. Please join us for this 'Lunch and Learn' presentation that will focus on the latest technical advances in AFM that enable high resolution imaging of the structure and dynamics of samples including proteins, lipids and nucleic acids. We’ll show examples of how the Cypher ES Environmental AFM allows users to control the environment around their sample and perform perfusion experiments easily. You’ll learn about Cypher’s numerous ease-of-use features such as GetStarted™, GetReal™, and blueDrive™ for easy and stable imaging in liquid. We will introduce you to Fast Force Mapping, our unique technology that measures mechan- ical properties of your samples faster and more reliably. This is also a great opportunity to ask our scientists any questions you may have about AFM. Presenter Irène Revenko, Applications Scientist, Asylum Research, an Oxford Instruments Company

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Biophysical Society 59 th Annual Meeting, Baltimore, Maryland