Biophysical Society 59th Annual Meeting Program Guide

924-P lat 10:00 am USING LONG-TIMESCALE MOLECULAR DYNAMICS SIMULATIONS TO BENCHMARK ENHANCED SAMPLING METHODS. Albert C. Pan , Thomas M. Weinreich, Stefano Piana, David E. Shaw

8:15 am –10:15 am , R oom 316/317 Platform Micro- and Nanotechnology

Co-Chairs Aleksandra Radenovic, Ecole Polytech Federal Lausanne, Switzerland Sergey Bezrukov, NIH/NICHD 933-P lat 8:15 am PROBING THE MOTION OF THE INTRINSICALLY DISORDERED NEURONAL PROTEIN ALPHA-SYNUCLEIN THROUGH THE VDAC PORE USING A SINGLE-MOLECULE APPROACH. David P. Hoogerheide , Philip A. Gurnev, Tatiana K. Rostovtseva, Sergey M. Bezrukov 934-P lat 8:30 am USING NANOPARTICLES TO CONTROL CELLULAR MEMBRANE POTENTIAL. Emilie A.K. Warren , Christine K. Payne 935-P lat 8:45 am ELECTRO-WETTING OF A HYDROPHOBIC GATE IN A BIOMIMETIC NANOPORE. Jemma L. Trick , Chen Song, Jayne E. Wallace, Hagan Bayley, Mark S P Sansom 936-P lat 9:00 am DEFORMATION OF MCF-7 CELLS IN MICROPORES WITH UNDULATING DIAMETER. Laura M. Innes , Ashley Fong, Matthew Pevarnik, Matthew Schiel, Eugenia Toimil-Molares, Luke Theogarajan, Christopher Hughes, Zuzanna Siwy 937-P lat 9:15 am FINGERPRINTING SINGLE LIVING CELLS WITH MOLECULAR PRECISION. Kim McKelvey , Volker Kurz, Tetsuya Tanaka, Gregory Timp 938-P lat 9:30 am MECHANICAL MODULATION OF ENZYME ACTIVITY BY RATIONALLY DESIGNED DNA TWEEZERS: FROM THE ENSEMBLE TO THE SINGLE-MOLECULE LEVEL. Soma Dhakal , Minghui Liu, Matthew R. Adendorff, Mark Bathe, Hao Yan, Nils G. Walter 939-P lat 9:45 am REGULATION OF LIPID MEMBRANE TRAFFICKING AND TRANSMEMBRANE SIGNALING BY GRAPHENE. Kristina E. Kitko , Tu Hong, Roman Lazarenko, Da Ying, Yaqiong Xu, Qi Zhang 940-P lat 10:00 am DEVELOPMENT OF A FLUORESCENCE-BASED ASSAY FOR FUNCTIONAL STUDIES OF TRANSPORTER PROTEINS ON THE SINGLE MOLECULE LEVEL. Salome Veshaguri, Sune M. Christensen, Mads P. Møller , Garima Ghale, Christina Lohr, Andreas L. Christensen, Marijonas Tutkus, Gerdi Kemmer, Ida L. Jørgensen, Bo H. Justesen, Patricia Curran, Thomas G. Pomorski, Joseph Mindell, Dimitrios Stamou

8:15 am –10:15 am , R oom 314/315 Platform Protein Dynamics and Allostery I

Co-Chairs Ian Thorpe, University of Maryland, Baltimore Lukas Stelzl, University of Oxford, United Kingdom 925-P lat 8:15 am

ALLOSTERIC COMMUNICATION WITHIN THE CYTOPLASMIC REGION OF THE HISTIDINE KINASE CPXA, REVEALED BY MOLECULAR DYNAMICS SIMULATIONS OF THE WILD-TYPE AND M228V PROTEINS. Marlet Martinez , Nathalie Duclert, Jean- Michel Betton, Pedro M. Alzari, Michael Nilges, Thérèse E. Malliavin 926-P lat 8:30 am STUDYING THE CONFORMATIONAL EQUILIBRIUM OF THE N-TERMINAL DOMAIN OF DSBD BY NMR AND COMPUTER SIMULATION. Lukas S. Stelzl , Despoina A.I. Mavridou, Stuart J. Ferguson, Andrew J. Baldwin, Mark S.P. Sansom, Christina Redfield 927-P lat 8:45 am FUNCTIONAL MECHANISM OF THE PHOTOACTIVE YELLOW PROTEIN: A TRANSIENT ABSORPTION SPECTROSCOPY PERSPECTIVE. Chandra P. Joshi , Harald Otto, Maarten P. Heyn 928-P lat 9:00 am DUAL ALLOSTERIC INHIBITORS EXHIBIT ANTAGONISTIC EFFECTS IN THE HEPATITIS C VIRUS POLYMERASE. Jodian A. Brown, Ian F. Thorpe 929-P lat 9:15 am MICROSECOND MOTION MODULATES UBIQUITIN BINDING THROUGH AN ALLOSTERIC BACKBONE/SIDE CHAIN NETWORK. Colin A. Smith , David Ban, Karin Giller, Stefan Becker, Christian Griesinger, Donghan Lee, Bert L. de Groot 930-P lat 9:30 am ALLOSTERY IN PDZ3: USING UNNATURAL AMINO ACIDS AS SITE-SPECIFIC REPORTERS IN IR SPECTROSCOPY TO PROBE ALLOSTERIC PATHWAYS. Katharina B. Eberl , Henrike M. Müller- Werkmeister, Martin Essig, Jens Bredenbeck 931-P lat 9:45 am ALLOSTERIC COMMUNICATION IN RND1 AND RAC1 ASSOCIATION WITH THE PLEXIN-B1 RHOGTPASE BINDING DOMAIN REVEALED BY HYDROGEN EXCHANGE MASS SPECTROSCOPY AND BY SOLUTION NMR. Shufen Cao , Matthias Buck 932-P lat 10:00 am CONFORMATIONAL TRANSITIONS IN SWITCH REGIONS OF THE RAS-LIKE GTPASE RAB1B STUDIED BY FREE ENERGY SIMULATIONS. Manuel Patrick Luitz , Rainer Bomblies, Martin Zacharias, Evelyn Bender, Aymelt Itzen

8:30 am –10:00 am , H all C, R oom B Exhibitor Presentation FEI Company Advances in Correlative Light and Electron Microscopy

Correlative light and electron microscopy (CLEM) is a powerful approach that enables combining dynamic information and labelling specificity from fluorescence microscopy with ultra-structural information at nanometer resolution from electron microscopy on the same sample. In recent years technical improvements in fluorescence microscopy have enhanced z-resolution, enabled imaging with high sensitivity using TIRF

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Biophysical Society 59 th Annual Meeting, Baltimore, Maryland