SPADA

Challenges ‐ 10 species currently described

Many demonstrate host specificity

Existing Methods • Foster et al. 2008 SNP‐based real‐time PCR assays  f ll j B ll l d or a ma or ruce a c a es • Fretin et al. 2008 SNP‐based real‐time PCR assays  for B. suis (137 field strains) • Hinić et al. 2008 Conventional and real‐time PCR  assays for multiple Brucella species • Lopez‐Goñi et al. 2016 Multiplex “Bruce‐ladder” for all known species • Scholl et al., 2010 Multiplex PCR assay for all  described species • Yu and Nielsen, 2010 Croatian Med J 51 review