SPADA

NA TEST VALIDATION Iterative approaches to test validation

Previous validations: Sensitivity analysis: Serial 100-fold dilutions (1ng/ µ l to 1 fg/ µ L) of DNA Inclusivity panel – Variola virus DNA: 2 purified viral stocks 33 crude viral stocks 2 Human scab samples Specificity analysis: Serial 100-fold dilutions (1ng/ µ l to 1 fg/ µ L) of DNA Exclusivity panel – near neighbor ( Orthopoxvirus ): Eurasian: 1 Ectromelia virus

2 Monkeypox viruses 2 Camelpox viruses 1 Cowpox virus

1 Taterapox virus 5 Vaccinia viruses North American: 1 Skunkpox virus Exclusivity panel – other rash-causing illnesses: 1 Varicella Zoster virus 1 Herpes simplex virus (type 1) 1 Rickettsia strain Exclusivity panel – negatives: Myxoma & tissue culture (2)