SPADA

Inclusivity Panel

Species

Strain

Sample

Complementary set: Exclusivity Panel - near neighbor ( Orthopoxviruses ) Species Strain Ectromelia virus Moscow Monkeypox virus 79-0266 Monkeypox virus 79-0005 Camelpoxvirus LLC Camelpoxvirus V78-I-903 Cowpoxvirus Brighton Taterapoxvirus (Gerbilpox) Vaccinia virus Lister Vaccinia virus VTH Vaccinia virus Wyeth Vaccinia virus WYH pGS62-9-v1-1-1 Vaccinia virus Rabbitpoxvirus Skunkpovirus Exclusivity Panel - other rash-causing illnesses Species Strain Varicella Zoster Virus Webster Herpes Simplex Virus-1 HFEM Rickettsia conorii

Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus Variola virus

102 103

Crude Crude Crude Crude Crude Crude Crude Crude Crust Crude Crude Crude Crude Crude Crude Crude Crude Crude Crude Crude Crude Crude Crude Crude Crust Crude Crude Crude Crude Crude Crude Crude Crude Crude Crude Pure Pure

66-39 7124 7125

72-119 73-175 77-1605 Bombay Congo Eth-17 Harper Harvey

Brazil-Garcia

Heidelberg

Higgins Hinton

Horn Horn

K1629

Kali Mathu Kembula

Minnesota 124

MS Lee

Nepal

New Dehli

Nigeria Kuclano

Nur Islam Rumbec

Exclusivity Panel - other negatives

Shahzamon

Solaiman Stillwell V68-59 V70-222 V70-228

Species

Strain

Myxoma

Human tissue culture cells

Sup-T

Variolator-4

Monkey kidney tissue culture cells

BSC-40

Yamada

Validated diagnostic real- time PCR assays Target multiple regions of the genome Target sequences specific to Variola virus Cowpox virus sequences acquired after validation Variola virus : diagnostic creation and validation

Exhibit extensive phylogenetic diversity Contain certain regions previously thought to be specific to Variola virus • Now assays predicted to

cross-react with Cowpox virus

D.Carroll, et.al