SPADA

Background

Exclusivity panel: Strains picked and rationale for selection B th i i i i l t d i U S EPA d . ur ng ens s so a es use n . . -approve pesticides • Bacillus thuringiensis (Berliner) (no subsp. given)

• Bacillus thuringiensis subsp. aizawai • Bacillus thuringiensis subsp. israelensis • Bacillus thuringiensis subsp. kurstaki B ill th i i i b t b i i * • ac us ur ng ens s su sp. ene r on s

*aka subsp. morrisoni

0.5 0.6 0.7 0.8 Genetic Distance

Branch A 53 B. thuringiensis

B. thuringiensis subspecies israelensis B. thuringiensis subspecies morrisoni

Branch B 22 B. thuringiensis 2 B. cereus B. thuringiensis 10792 type strain

Cluster 1

Branch C 122 B. thuringiensis 17 B. cereus B. cereus 14579 type strain B. thuringiensis kurstaki33679

B. thuringiensis subspecies kurstaki B. thuringiensis subspecies aizawai

Branch D 6 B. cereus

Branch E 1 B. cereus

Cluster 2

Branch F 18 B. thuringiensis 18 B. cereus 25 B. anthracis

All B. anthracis Isolates map here

Branch G . urngenss 1 B. cereus i

7 B th i i

Branch H 10 B. thuringiensis 9 B. cereus

Cluster 3

Branch J 4 B. thuringiensis 1 B. cereus

From Hill, K.K., et al. (2004) Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus and B. thuringiensis isolates. Appl. Environ. Microbiol . 70, 1068-1080

Branch K 10 B. thuringiensis 6 B. cereus