SPADA

Sequenced near neighbors

Whole genome SNP phylogeny of  Francisella by the Swedish Defense Research Agency (FOI) 

SMPR Key Points

• In silico screening required – Purpose: Predict specificity and inclusivity  across all sequenced Francisella strains – Approach: Use Basic Alignment Search Tool (BLAST) to predict hybridization events  between signatures components (e.g., oligo  primers/probes/amplicons) and available  Francisella genomic sequences in GenBank – Include results of  in silico analyses in method/  assay performance evaluation reports