September 2015 SPADA Meeting

Bacillus anthracis Exclusivity Near Neighbors Number Species Strain Plasmid Status NN1 B. cereus S2‐8 pXO1‐, pXO2‐ NN2 B. cereus 3A pXO1‐, pXO2‐ NN3 B. thuringiensis HD1011 pXO1‐, pXO2‐ NN4 B. thuringiensis 97‐27 pXO1‐, pXO2‐ NN5 B. thuringiensis HD682 pXO1‐, pXO2‐ NN6 B cereus E33L pXO1 pXO2 .  ‐,  ‐ NN7 B. cereus D17 pXO1‐, pXO2‐ NN8 B. thuringiensis HD571 pXO1‐, pXO2‐ NN9 Bacillus Al Hakam pXO1‐, pXO2‐ NN10 B. cereus ATCC 4342 pXO1‐, pXO2‐ NN11 B. cereus FM1 pXO1‐, pXO2‐ NN12 B. cereus G9241 pBCXO1+*, pXO2‐ NN13 B. cereus 03BB102 pXO1+**, capA+, capB+, capC+*** NN14 B. cereus 03BB108 pXO1+**, capA+, capB+, capC+*** NN15 B. thuringiensis subsp. Israelensis pXO1‐, pXO2‐ NN16 B. thuringiensis subsp. kurstaki pXO1‐, pXO2‐ NN17 B. thuringiensis Subsp. Morrisoni pXO1‐, pXO2‐ NN18 B. coagulans ATCC 7050 pXO1‐, pXO2‐ NN19 B. mycoides ATCC 6462 pXO1‐, pXO2‐ NN20 B. megaterium ATCC 12872 pXO1‐, pXO2‐

*pBCXO1 is pXO1‐like, but not identical. ** Not all of the pXO1 plasmid is present in these two isolates.   B. cereus 03BB102 is missing parts of the plasmid near ORF’s 12 through  37, near ORF’s 84 through 89 and near ORF 115 among others.   B. cereus 03BB108 is missing even more extensive regions of pXO1. ***capA, B and C are contained within the pXO2 plasmid of B. anthracis , and are found in B. cereus strains 03BB102 and 03BB108.

Environmental Panel

7 Agents

37 "Air and Soil" (continued) Clostridium perfringens Clostridium sardiniense Deinococcus radiodurans

5 Viruses

4 Plants

Brucella melintensis

Herpes simplex 1 3 Adenovirus 14

Pine Corn

Burkholderia pseudomallei Clostridium botulinum

Gypsy moth virus (Gypcheck)

Cotton

Coxiella brunetii

Delftia acidovorans

Vaccinia virus (pox)

Ricin

Yersinia pestis Colorado-92 Francisella tularensis Schu-S4

Escherichia coli

Cyd-X Product

9 Arthropods

Fusobacterium nucleatum Lactobacillus plantarum 2 Legionella pneumophila Listeria monocytogenes Moraxella nonliquefaciens Mycobacterium smegmatis Pseudomonas aeroginosa Rhodobacter sphaeroides 2 Riemerella anatipestifer Shewanella oneidensis 2 Staphylococcus aureus Stenotophomonas maltophilia Streptococcus pneumoniae Streptomyces coelicolor Neisseria lactamica

2 Amoebae

Cockroach

Rickettsia prowazekii 1 37 "Air and Soil" Acinetobacter lwoffii

Acanthamoeba castellanii

Aedes albopictus (mosquito)

Naegleria fowleri

Fruit fly

11 Fungi 4

Gypsy moth

Agrobacterium tumefaciens Bacillus benzoevorans

Alternaria alternata Aspergillus fumigatus Aureobasidium pullulans Cladosporium cladosporioides Epicoccum nigrum Eurotium amstelodami Mucor racemosus Paecilomyces variotii Penicillium chrysogenum

Tick Flea

Bacillus cohnii

House fly Dust mite

Bacillus horikoshii Bacillus macroides 2 Bacillus megaterium Bacteroides fragilis 2 Burkholderia cepacia Burkholderia gladioli Burkholderia plantarii Burkholderia stabilis

Cladosporium sphaerospermum Aedes aegypti (mosquito)

6 Mammalian/Avian Cell Lines

Bacillus psychrosaccharolyticus

Mouse

Rat Dog Cat

Wallernia sebi

Human Chicken

Synechocystis

Chryseobacterium indologenes

Vibrio cholerae

Clostridium butyricum 2

Total of 81 biological entities in the Environmental panel